Novel Immunomodulatory Flagellin-Like Protein FlaC in Campylobacter jejuni and Other Campylobacterales
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چکیده
منابع مشابه
Novel Immunomodulatory Flagellin-Like Protein FlaC in Campylobacter jejuni and Other Campylobacterales
The human diarrheal pathogens Campylobacter jejuni and Campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, FlaA and FlaB, have a low intrinsic property to activate the innate immune receptor Toll-like receptor 5 (TLR5). We have investigated here the hypothesis that the unusual secreted, flagellin-like molecule FlaC present in C. jejuni, C. co...
متن کاملStructural and functional analysis of two Campylobacter jejuni flagellin genes.
The purpose of this study was to characterize the flagellin gene of Campylobacter jejuni and to study the structure of this protein and the regulation of its synthesis. A part of the flagellin gene of C. jejuni strain 81116 was recently cloned by us. This DNA fragment was used as a probe to isolate the other homologous flagellin sequences from genomic libraries. The flagellin nucleotide sequenc...
متن کاملReconstitution of a functional Toll-like receptor 5 binding site in Campylobacter jejuni flagellin.
Bacterial flagellin is important for intestinal immune homeostasis. Flagellins from most species activate Toll-like receptor 5 (TLR5). The principal bacterial food-borne pathogen Campylobacter jejuni escapes TLR5 recognition, probably due to an alternate flagellin subunit structure. We investigated the molecular basis of TLR5 evasion by aiming to reconstitute TLR5 stimulating activity in live C...
متن کاملDistribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni.
The complex flagellar filaments of the LIO8 serogroup member Campylobacter coli VC167 are composed of two highly related subunit proteins encoded by the flaA and flaB genes which share 92% identity. Using oligonucleotide primers based on the known DNA sequence of both the flaA and flaB genes from C. coli VC167 in the polymerase chain reaction, we have shown conservation of both fla genes among ...
متن کاملReservoir competence of lesser mealworm (Coleoptera: Tenebrionidae) for Campylobacter jejuni (Campylobacterales: Campylobacteraceae).
The lesser mealworm, Alphitobius diaperinus (Panzer), is a carrier of Campylobacter spp. in poultry facilities; however, the beetle's importance in the epidemiology of campylobacteriosis is not known. A series of laboratory experiments were designed to test the vector and reservoir competence of the lesser mealworm for Campylobacter jejuni. In the first experiment, C. jejuni was swabbed onto th...
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ژورنال
عنوان ژورنال: mSphere
سال: 2016
ISSN: 2379-5042
DOI: 10.1128/msphere.00028-15